Senior/Research Associate, mRNA Engineering

About NewLimit
NewLimit is a biotechnology company working to radically extend human healthspan. We’re developing medicines to treat age-related diseases by reprogramming the epigenome, a new therapeutic mechanism to restore regenerative potential in aged and diseased cells. We leverage functional genomics, pooled perturbation screening, and machine learning models to unravel the biology of epigenetic aging and disease using experiments of unprecedented scale.
Position
We are seeking a Research Associate to join our mRNA Engineering team. In this role, you will generate the mRNAs that power NewLimit's research programs, executing the workflow from DNA template through in vitro transcription (IVT) and downstream purification. You will produce research grade mRNA materials, keep rigorous records of how each batch was made, and work closely with scientists across our research and Delivery Sciences teams. This is a lab-based role ideal for an early-career scientist who wants to build deep expertise in mRNA process development in a fast-paced research environment. You will receive hands-on training from expert scientists and see the direct impact of your work on our programs.
What you'll do
As a member of our team, you will:
mRNA production
Execute established protocols for DNA template preparation, in vitro transcription, and downstream purification to generate research-grade mRNA
Run routine analytical assays to assess mRNA integrity and purity
Under the guidance of senior scientists, help troubleshoot production issues and contribute to experiments that optimize yield, purity, and recovery
Documentation and lab operations
Maintain detailed, accurate records of all experiments in Electronic Lab Notebooks
Follow and help maintain SOPs; contribute to drafting and updating protocols as workflows evolve
Manage raw material and mRNA inventory, including sample tracking, storage, and reagent ordering
Maintain lab equipment and follow lab safety practices
Collaboration
Work closely with Delivery Sciences and research teams to coordinate material needs and hand off mRNA for downstream studies
Present experiment results at team meetings
Requirements
B.S. in Life Sciences, Chemistry, Engineering, or a related field with 1-2 years of relevant full-time industry or academic research experience, or M.S. in Life Sciences
Hands-on experience with molecular biology techniques such as cloning, nucleic acid purification, PCR, and gel electrophoresis
Strong attention to detail and good documentation practices
Ability to execute protocols accurately and manage multiple tasks efficiently
Excellent communication and organizational skills
Interest in mRNA process science and eagerness to learn new techniques
Nice to have
Experience producing mRNA through IVT and downstream purification
Familiarity with plasmid and mRNA analytics such as HPLC, UV-Vis, and TapeStation/CGE
Experience with plasmid DNA preparation and linearization
Exposure to column-based purification and tangential flow filtration (TFF)
Familiarity with automation liquid handling-based methods for mRNA purification, including experience operating such as Hamiltons and Tecans
Benefits
NewLimit offers generous benefits to ensure our team can focus on our mission:
Health, dental, and vision insurance
401(k) with company match
Paid parental leave
Industry-leading paid time off (PTO) — 20 days/year for all employees + holidays
Meals and snacks on-site
Salary Range: $75,000 - $100,000*. Compensation for the role will depend on a number of factors, including a candidate’s qualifications, skills, competencies, and experience. NewLimit offers a competitive benefits package, which includes a 401k, healthcare coverage, and 20 days of PTO. Learn more at: https://www.newlimit.com/careers
*This compensation and benefits information is based on Company's good faith estimate as of the date of publication and may be modified in the future.