Research Assistant

The Yellen lab at Harvard Medical School Neurobiology is looking for a research technician. Our research is focused on understanding neuronal metabolism and how it is influenced by – and influences – neuronal activity and excitability. The lab develops tools for studying neural metabolism, including engineered proteins that function as fluorescent biosensors of metabolism, and we study the dynamics of metabolism in mouse brain using both ex vivo and in vivo imaging methods. We also work to learn the role of altered metabolism in preventing epileptic seizures, inspired by the ability of ketogenic diets to treat human epilepsy.
The successful candidate will work under close supervision by Dr Yellen and others in the lab to:
1) manage a moderate size mouse colony, including genotyping and performing mouse surgery (stereotactic injection of viral expression vectors);
2) perform molecular biology procedures including plasmid DNA preparation, PCR, subcloning, biochemical assays, bacterial expression of fluorescent proteins, and fluorescence analysis; and
3) perform a small amount of tissue culture (cell line maintenance and transfection).
There may also be the opportunity to do semi-independent projects on fluorescent biosensor development and/or biosensor imaging. The successful candidate should be well-organized, motivated, and a responsible team member.
Basic Qualifications:
At least one-year related work experience (relevant course work may count towards experience).
College background or equivalent work experience, preferably in a related discipline.
Additional Qualifications and Skills:
The candidate should have a general education in molecular biology and should be familiar with the basic concepts of plasmids, expression vectors, and subcloning, as well as hands-on experience with good laboratory practices and sterile technique.
Some experience with mice is preferred.
Detailed guidance and training will be provided for each of the specific procedures used. All of the work requires attention to detail, and some of it requires creative problem solving.
In general, the successful candidate is someone who always has their “head in the game.”

Employment type
Full-time

Work arrangement
No